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Icount 33 Automatic Colony Counter
Product Description
Adopting an all-metal chassis without ambient light interference, the multi-mode illumination system integrates fog light diffuse reflection illumination, white ling translucent bottom light illumination, and compound suspended dark field illumination to adapt to various types of flat dish imaging.
The software is equipped with rich image recognition algorithms, suitable for complex colony image processing. The new version of the software has added a fast statistics module, customers just need to rotate the mouse wheel, easy to get the best statistical results, lifting the laborious manual parameter setting and trying.
Fog light diffuse lighting
The fog light system consisting of 96 LED arrays and nano light-reflecting materials is embedded in the design, and the bright, foggy diffuse light fills the camera chamber to form a uniform upper illumination. Colonies and culture media have a natural color, and glass petri dishes do not form common refraction phenomena such as light spots and halos.
Duplex Suspended Darkfield Lighting
The compound dark field illumination consists of a double layer of dark field contour light with a black background, and the light intensity can be freely adjusted. The uniform, parallel mixed backlight penetrates the culture medium and gives a beautiful contour to the colonies, creating brightly colored colonies against a dark background.
white translucent underlighting
Xun digital original Ling translucent backlighting lighting, composed of white LED backlight module light source, reflective plate, light guide plate, you can control the intensity and direction of the transmitted light. A change in the conventional backlighting lighting can only form a silhouette mode, for light colors, tiny colonies more three-dimensional expression.
Highly efficient digital imaging
High-fidelity fixed-focus lens, 8.5 megapixel USB3.0 high-speed CMOS industrial camera to quickly show colony morphology and color.
Intelligent and fast statistics
Seven kinds of fast statistical buttons, easy to use, just the mouse wheel rotation to achieve continuous parameter changes, easy to get the best statistical results.
Eight complex statistical algorithms to adapt to the diversity and complexity of the plate, such as multiple adhesions, uneven media, coexistence of impurities and colonies, diffusion of pigments, and proximity of colonies to the color of the media ......
Product Specification
I. Lighting system
Ø Fully enclosed steel-aluminum alloy chassis (32×34×46cm): precise and sturdy to ensure light confinement
Ø Flat dish sample-carrying compartment: aluminum alloy frame, drop-down partition window, eliminating environmental stray light interference, blocking UV leakage, avoiding dust entry.
Backlighting
High-density white LED array creates uniform, bright white transmitted light, ensuring even illumination around the edges and in the center of the petri dish.
Ø Duplicate suspended darkfield lighting
White LEDs intertwined and mixed with blue LEDs, broadband backlighting, forming a cosmic blue background
Ø Matte diffuse illumination
1. 96 LED arrays and nano light reflective materials constitute an embedded fog light system, 360°continuous diffuse reflection, highlighting the color and texture of the colony, eliminating the refraction of the glass Petri dish formed by the light spot, halo.
2. Color temperature range: 3100K-5800K Illumination range 50--7000 Lux
3. LED life time≥20000 hr
Ø UV light source: 254nm for cavity sterilization, UV mutagenesis
Ø Light source controller
1. Invisible pop-up control panel, 5-channel lighting selector switch, 4-channel stepless brightness adjustment, dual-channel color temperature adjustment
2. Freedom to switch and select single-mode lighting or combined-mode lighting
II. Digital imaging
Ø SD Industrial Fixed Focus Lens: 8mm, 5.0 mega-pixel, 2/3", Distortion <1%, F1.4 to F16, C-Mount
Ø Professional CMOS camera: chip size 1/2.4"; CMOS physical pixels 8.5 million, 3328x2548; individual pixel size 1.67x1.67µm
III. Colony analysis module
1. Rapid colony count
Ø Wheel parameter adjustment statistics (4 types): homogeneous dish, uneven background, microcolony, colored background
Ø One-click response statistics (3 types): monochrome statistics, mold statistics, trans statistics
1. Advanced colony counting
Ø Dynamic adjustment of statistics: the statistical results can be dynamically adjusted and corrected to quickly obtain the best statistical results.
Ø Deviation prediction statistics: suitable for colonies with many and complex colors.
Ø Horizontal set multi-model algorithm: search operation to obtain the best image segmentation effect and adapt to media background transformation
Ø Specific colony statistics: Identify specific colonies based on their color, size, and contour characteristics.
Ø Trans statistics: suitable for extremely complex colony types with a homogeneous medium background
Ø Fungus, impurity rejection: according to the difference in shape, size, color, automatic fungus, impurity rejection
2. Basic colony counting function
Ø Flat dish type: pouring, coating, membrane filtration, 3M paper chips
Ø Whole dish colony statistics: total colony counts and display by 25 sizes.
Ø Area selection statistics: can select the circle, rectangle, any circled area for statistics.
Ø Multi-domain parallel statistics: one-time multi-region synchronized statistics; multi-region "skeleton" statistics
Ø Diameter classification statistics: set the diameter range, statistics of specific size colonies
Ø Mouse click counting: quickly mark and add colonies, suitable for counting colonies at the edge of the petri dish
Ø Colony adhesion segmentation: automatic segmentation of mutually adhesive colonies, chained colonies can be selected by the user to be segmented or not.
3. Mesh filter membrane with 3M test piece
Ø Solid black grid for one-click statistics
Ø 3M Total Bacteria Test Piece, 3M Staphylococcus aureus Test Piece: One Click Statistics
Ø 3M Coliform Test Piece, 3M E. coli/Coliform Rapid Test Piece: one-click statistics + manual selection
4. Advanced tools
Ø Grid Removal: Eliminate filter membrane grid background interference
Ø Manual counting correction: adding or deleting colonies
Ø Exclude contaminated area: mouse outline any contaminated area, automatically exclude the number of colonies in the contaminated area.
Ø Background text elimination: automatic elimination of marker interference
Ø Manual Adhesion Segmentation: manual segmentation of multiple adherent colonies
Ø Parameter automatic conversion: Petri dish diameter, sample dilution input, realize automatic conversion
Ø Text, graphic annotation: various drawing tools and Chinese and English text embedding
5. Calibration and measurement
Ø Instrument calibration: Instrument self-calibration, manual correction calibration
Ø One-button fast measurement: one-button determination of large colonies, suitable for single colony analysis of fungi, actinomycetes
Ø Whole dish automatic measurement: equivalent diameter, area, long and short diameter, circumference, roundness analysis of whole dish colonies
Ø Multi-directional scale measurement, manual precise measurement: length, angle, radian, area, arc, arbitrary curve
IV. Data security and management
Ø "Management, operation, review" multiple structures, divided into functions and permissions, to ensure data security, integrity and authenticity
Ø Single-dish data recording: measured colony number, area-converted colony number, dilution-converted colony number, morphological parameters of each colony, size-graded statistics, regional statistics.
Ø Electronic data logging: source of sample, number, dilution, picture of flat dish, identification effect, count value, statistical tools used, parameter settings, corrections
Ø Electronic data is automatically stored or printed out in PDF or Excell format.
V. Instrument Specifications and Configuration
Ø icount 33 mainframe 1 unit
Ø Colony analysis software
Ø Branded business desktop computer: dual-core CPU/4G RAM/1T hard disk/21.5" HD screen, Windows 10 system